The Effect of Spinal Manipulative Therapy
on Experimentally Induced Pain:
A Systematic Literature Review

This section is compiled by Frank M. Painter, D.C.
Send all comments or additions to:

FROM:   Chiropractic & Manual Therapies 2012 (Aug 10);   20 (1):   26 ~ FULL TEXT

Mario Millan, Charlotte Leboeuf-Yde, Brian Budgell, and Michel-Ange Amorim

University Paris-Sud,
Paris, France.

BACKGROUND:   Although there is evidence that spinal manipulative therapy (SMT) can reduce pain, the mechanisms involved are not well established. There is a need to review the scientific literature to establish the evidence-base for the reduction of pain following SMT.

OBJECTIVES:   To determine if SMT can reduce experimentally induced pain, and if so, if the effect is

i) only at the level of the treated spinal segment,

ii) broader but in the same general region as SMT is performed, or

iii) systemic.

DESIGN:   A systematic critical literature review.

METHODS:   A systematic search was performed for experimental studies on healthy volunteers and people without chronic syndromes, in which the immediate effect of SMT was tested. Articles selected were reviewed blindly by two authors. A summary quality score was calculated to indicate level of manuscript quality. Outcome was considered positive if the pain-reducing effect was statistically significant. Separate evidence tables were constructed with information relevant to each research question. Results were interpreted taking into account their manuscript quality.

RESULTS:   Twenty-two articles were included, describing 43 experiments, primarily on pain produced by pressure (n = 27) or temperature (n = 9). Their quality was generally moderate. A hypoalgesic effect was shown in 19/27 experiments on pressure pain, produced by pressure in 3/9 on pain produced by temperature and in 6/7 tests on pain induced by other measures. Second pain provoked by temperature seems to respond to SMT but not first pain. Most studies revealed a local or regional hypoalgesic effect whereas a systematic effect was unclear. Manipulation of a "restricted motion segment" ("manipulable lesion") seemed not to be essential to analgesia. In relation to outcome, there was no discernible difference between studies with higher vs. lower quality scores.

CONCLUSIONS:   These results indicate that SMT has a direct local/regional hypoalgesic effect on experimental pain for some types of stimuli. Further research is needed to determine

i) if there is also a systemic effect,

ii) the exact mechanisms by which SMT attenuates pain, and

iii) whether this response is clinically significant.

From the FULL TEXT Article:



Pain is defined as an unpleasant sensory and emotional experience associated with actual or potential tissue damage [1]. It originates in specific receptors, named nociceptors, which are classified according to the type of damage to which they respond; thus, mechanosensitive, thermosensitive, chemosensitive and polymodal nociceptors.

From the peripheral nociceptors, noxious stimuli are transmitted to the dorsal horn of the spinal cord [2]. There, afferent fibers synapse in the superficial laminae of the dorsal gray matter of the spinal cord [3]. Cells in the superficial laminae serve as an integration centre and relay system for many sensations. Most cells of the grey matter involved in nociception send axons across the midline of the spinal cord to ascend to the thalamus. From there, they project upwards, eventually to the cortex of the brain. However, impulses are not only transmitted to the cerebral cortex. There are also other pathways and mechanisms that may participate in analgesic influences at the spinal and supraspinal levels.

When a noxious stimulus occurs, there may be a “first pain”, conducted by Aö fibers, and a “second pain”, due to temporal sensory summation (TSS) and conducted by nociceptive C-fibers. “First pain” is described as sharp and "pricking". The propagation of this stimulus is relatively quick and it is felt in a well-defined part of the body surface [2]. “Second pain”, which is transmitted more slowly, is often described as dull and aching, and it is poorly localized. This pain tends to last beyond the termination of an acute noxious stimulus. Sources, pathways, perception of and treatment of the two types of pain are very different [4].

      Modulating pain

The infliction of pain is not always experienced in a linear manner according to the strength and nature of the stimulus. Pain sensations can be very different from one individual to another, and also intra-individual variations can occur, so that identical types of damage do not necessarily result in an identical amount and type of pain. One reason for this is that pain can be modulated, both to increase and decrease.

One modulating system, central sensitization, tends to increase pain sensation [5], particularly in people who have more long-lasting pain, making them more sensitive to “new” pain impulses than they would have been under normal circumstances.

Another endogenous modulating system is afferent or segmental inhibition, meaning that one external stimulus can block an ongoing pain sensation by having higher priority in reaching the brain [4].

Descending antinociceptive systems provide yet another modulating mechanism. These originate largely in the mesencephalon and have synaptic connections with neurons in the medulla and the spinal cord. This means that nociceptive information may be blocked or attenuated before it reaches higher centers [6]. This system is also tightly connected to a descending pain facilitating pathway that has the same general sites of origin (mesencephalon and medulla) but with the opposite effect.

Finally, there are also other intrinsic mechanisms for physiological modulation of pain, such as subjective assessment and motivational-affective modulation [7], which act by raising pain thresholds via endogenous opioids and other substances. These mechanisms, at times, preferentially alter sensory and/or affective aspects of pain perception, and the associated modulation of pain-evoked neural activity occurs in limbic and/or sensory brain regions, suggesting multiple endogenous pain-modulating systems [8]. Thus, pain can be increased or decreased by mere expectations or, even, abolished by feelings of, for example, fear. Treating back pain with spinal manipulative therapy

Although back pain is common and frequently distressing, and many therapies have arisen to treat it, musculoskeletal pain remains difficult to diagnose and treat. Spinal manipulative therapy (SMT) is one common treatment for musculoskeletal pain. One class of SMT involves a high-velocity, low-amplitude (HVLA) manipulation frequently used by chiropractors. HVLA treatments are mechanical events. They cause slight momentary deformations of the spine and surrounding soft tissues, and often elicit a cracking sound thought to be brought about by cavitation of spinal facet joints [9-11]. It is common to differentiate manipulation from mobilization. In the latter case, the joint is not taken beyond its passive limit. Rather mobilization can be described as a passive and perhaps repetitive stretch. Manipulation, on the other hand, carries the vertebrae beyond the normal physiological range of movement without exceeding the boundaries of anatomic integrity [11]. However, the distinction between manipulation and mobilization is probably not that clear, and it has been shown that cavitation is not necessary for SMT to exert a clinical effect [12-16]. The term SMT can therefore be used to describe various types of manual therapy (MT).

      The possible mechanisms of spinal manipulative therapy in back pain

Clinical experience indicates that both HVLA and mobilization, and also other types of manual therapy (MT), can have an immediate effect on pain. The literature also suggests that SMT has a direct neurological pain-reducing effect, by evoking one or possibly several of the physiologic pain-modulating mechanisms described briefly above. Indeed, there could be a combination of mechanisms or a number of these acting on different causes of pain. In this article we shall concentrate on the possible direct effect of SMT on pain. There are three possible levels of this hypothesized direct effect of SMT on pain, i.e. local, regional or central.

      Local pain reducing effect

One theory is that SMT would have a pain-reducing effect primarily at the level of the manipulation, i.e. at a specific spinal level. In all, this phenomenon would probably be the result of a mix of different mechanisms. For example, SMT may mechanistically act to decrease the sensitivity of the muscle spindles and/or the various segmental sites of a reflex pathway [17].

      Regional pain reducing effect

Another possibility is that SMT could have a regional effect, although still at the spinal level of the manipulative input. Some authors suggest an effect on the dorsal horn of the spinal cord [18] or on the periaqueductal grey area [19-21]. SMT is also thought to affect reflex neural outputs to both muscle and visceral organs by affecting both paraspinal muscle reflexes and motoneuron excitability [22].

      Central pain reducing effect

Recently, it has been hypothesized that SMT reduces the potential for central sensitization by inhibiting TSS (“second pain”) [23]. One mechanism underlying the effects of SMT may be the ability of manipulation to alter central sensory processing by removing subthreshold mechanical or chemical stimuli from paraspinal tissues [22].

      Conflicting literature

Also a comprehensive model of mechanisms of manual therapy in the treatment of musculoskeletal pain has been suggested [24] consisting of a cascade of neurophysiological responses from the peripheral and central nervous systems which are then responsible for the clinical outcomes. In other words, the literature offers many possible mechanisms and combinations of mechanisms to explain the pain-reducing effect of SMT. In fact, there is a lot of information available in the literature on this topic. However, the literature is difficult to grasp and conflicting because it consists of a mixture of discussions, hypotheses, and studies employing different designs, methods and outcome variables. Therefore, there is a need for systematic and critical literature reviews in order to establish the evidence-base for various theories relating to the direct or indirect reduction of pain following SMT. A first step might be to establish the weight of evidence in relation to whether pain is indeed dampened by the application of SMT to the spinal structures.

      Aims and objectives

Therefore, in this systematic critical literature review we shall examine the effect of spinal manipulation on experimentally induced pain in healthy study subjects concentrating on the possible direct effects of SMT on pain at three levels, i.e. local, regional or systemic. Because different types of pain may travel through different pathways, these were studied separately.

The specific research questions were:

  1. Does SMT reduce pain provoked by pressure?

  2. Does SMT reduce pain provoked by temperature?

  3. Does SMT reduce pain provoked by methods other than pressure and temperature?

  4. Does SMT reduce experimentally induced pain at the spinal segment where it is performed?

  5. Does SMT reduce experimentally induced pain in the spinal region where it is performed?

  6. Does SMT have a systemic (global) effect on experimentally induced pain?


In order to obtain answers to the questions above, we undertook a systematic critical literature review, which commenced with a systematic literature search of PubMed, Mantis, and the Cochrane Library using specific search terms. These search terms were: Spinal manipulation pain: ("manipulation, spinal"[MeSH Terms] OR ("manipulation"[All Fields] AND "spinal"[All Fields]) OR "spinal manipulation"[All Fields] OR ("spinal"[All Fields] AND "manipulation"[All Fields])) AND ("pain"[MeSH Terms] OR "pain"[All Fields]) ; Chiropractic manipulation pain:("manipulation, chiropractic"[MeSH Terms] OR ("manipulation"[All Fields] AND "chiropractic"[All Fields]) OR "chiropractic manipulation"[All Fields] OR ("chiropractic"[All Fields] AND "manipulation"[All Fields])) AND ("pain"[MeSH Terms] OR "pain"[All Fields]) ; Spinal manipulation experimental pain: ("manipulation, spinal"[MeSH Terms] OR ("manipulation"[All Fields] AND "spinal"[All Fields]) OR "spinal manipulation"[All Fields] OR ("spinal"[All Fields] AND "manipulation"[All Fields])) AND experimental[All Fields] AND ("pain"[MeSH Terms] OR "pain"[All Fields]) ; and Chiropractic manipulation experimental pain: ("manipulation, chiropractic"[MeSH Terms] OR ("manipulation"[All Fields] AND "chiropractic"[All Fields]) OR "chiropractic manipulation"[All Fields] OR ("chiropractic"[All Fields] AND "manipulation"[All Fields])) AND experimental[All Fields] AND ("pain"[MeSH Terms] OR "pain"[All Fields]).

Inclusion and exclusion criteria (see Additional file 1 below) were applied by the first author to the titles and abstracts of the studies identified in the search. Once most selected articles were retrieved, a citation search was made based on the retrieved articles’ reference lists. All articles selected were reviewed separately by two different authors blinded to each other’s evaluations. Each author separately extracted data from every article according to a checklist. Data were later compared in order to minimize extraction errors. The fourth author would arbitrate any disagreement between the two reviewers. An ongoing search was performed until December 31, 2011 and the review process was repeated when new articles were found.

A table was constructed in order to describe the selected articles, as shown in Table 1.

Table 1.   Descriptive items used in a systematic critical literature review on the effect of SMT on pain

Articles are presented consecutively by year of publication and identified by a number corresponding to its reference in the first column of each table. As we were unable to locate a suitable quality check-list for this type of research, a second set of criteria was developed in order to evaluate the quality and risk of bias in this type of research. We designed this checklist based on concepts presented in the PRISMA statement [25], the CONSORT statement [26] and Cochrane guidelines [27] bearing in mind that there can be no general recipe for such work, as review procedures have to be topic specific. The items selected for the quality checklist and their rationale have been described in Additional file 2. The risk of bias was assessed following the criteria suggested by the method guidelines for systematic reviews of trials of treatments for neck and back pain by Furlan et al [27] (see items 1, 2, 4, 6, 9, and 11 in Additional file 2). Additional items were mainly adapted from PRISMA [25] (see items 3, 5, 7, 8, 10, 12, and 13). Finally, the outcomes were noted for each experiment as a positive effect, negative effect, or no effect. Classifying articles by their quality

Each article selected was checked for each quality item. By consensus, we decided to give one point for every criterion, except for the 4 items that we considered as most important: a- unbiased/blind/naïve study subjects (item 1), b- random allocation (item 3), c- blinded assessment (item 9), and d- losses and exclusions (item 11). Each of these was assigned two points.

A summary quality score was calculated but no cutoff-point was defined for acceptable or unacceptable level of quality. This allowed us (and the readers) to use the quality scores and the information on each individual quality item as a guide for whether articles would be considered more or less credible. In other words, the quality assessment was meant to be flexible.

A post hoc comparison was made between results of the two quality scores (the total score and the score for the most important criteria). Using thresholds set at 12/18 and 4/8 points, respectively, the distributions of studies with positive and non-positive outcomes were compared for the two scales and in relation to whether the studies were of higher or lower quality.

      Data synthesis

The data tables were used, in a systematic fashion, to obtain answers to our research questions. This was done by highlighting relevant information to facilitate a visual representation (green = positive outcome, red = non-positive outcome) to make interpretation easier. Finally, studies were sorted in descending order starting with those with the highest total quality score out of 18. Results were thereafter interpreted and reported in a narrative fashion. If high and low quality studies generally obtained similar outcomes, we assumed that the poorer quality studies provided supporting evidence for the better studies. If, however, it was mainly the poorer quality studies which obtained positive findings, particularly if the assessors were not blinded or the study subjects could have been biased, then we would be more cautious in our interpretation of the results.

Because we decided not to use a scoring system to establish level of quality, we have not defined discrete levels of evidence.


In all, 1279 titles satisfying the inclusion criteria were identified in the initial PubMed search. Other database search results were as follows:

a - Spinal manipulation pain: 1276 results in Pubmed, 10 in Mantis and 9 Cochrane Reviews.

b - Chiropractic manipulation pain: 806 results in Pubmed, 2 in Mantis and 5 Cochrane Reviews.

c - Spinal manipulation experimental pain: 63 results in Pubmed, 0 in Mantis and 13 Cochrane Reviews.

d - Chiropractic manipulation experimental pain: 28 results in Pubmed, 0 in Mantis and 0 Cochrane Reviews.

Only 5 articles were added after an additional search of reference lists.

All articles found in the Mantis and Cochrane databases were already identified via the Pubmed searches. Of the 1279 references, 116 were retrieved in full text for further scrutiny.

      Description of studies

Upon scrutinizing the full texts, 22 articles were found to fulfill the inclusion criteria. They described 43 experiments: 27 with pain produced by pressure, 9 by temperature, 3 by capsaicin, 2 examined spontaneous pain, 1 used a stretch test to produce pain and 1 used electrically induced pain. All were controlled trials published in English. Detailed information is presented in Table 1 and briefly summarized below.

Three research groups were responsible for 13 publications. They did not seem to have repeatedly used the same study samples but appeared to have reported on different study populations for the various studies. In most cases (n = 18), experiments were carried out totally or partially in universities. The number of subjects ranged from 10 to 122. Six articles used patients as study subjects; most of the others included non-clinical populations, often students. There were no studies with animals fulfilling our inclusion criteria.

Most of experiments (n = 20) used external control groups. Twelve of them used three groups: a treatment, a placebo and a control group in which no action was taken - noted in tables as “nothing” (studies [21, 23, 28-35]). Studies [18, 36] used bicycle exercise as their control activity. Seven studies ([19, 20, 37-41]) used a sham procedure as their control treatment, whereas study [42] compared the treatment group to a control group without a sham procedure. Two studies used only internal control groups; [43] and [44] compared three different types of mobilization.

      Data synthesis: Quality of studies

There were no disagreements between reviewers on the scoring of the manuscripts based on the checklists. Table 2 summarizes the quality items for each article. The quality and risk of bias assessment of studies reviewed revealed them to be relatively homogeneous, with the summary scores ranging between 8 and 16 out of 18 points, with mean and median summary scores of 12.2 and 13, respectively.

Table 2.   Quality criteria of articles selected for a systematic critical literature review on the effect of SMT on pain

Of the various quality items that we scrutinized, all studies fulfilled the criteria on the validity of the outcome variable, all had measured the pain before and after the intervention, all had provided some estimate of the results in tables, graphs or text, and all had tested their results for statistical significance. The ANOVA was used for factorial designs in all studies in order to test how variables interacted or combined.

Other checklist items were not always fulfilled. Of the four quality criteria that we considered particularly important, namely unbiased/blind/naïve study subjects (item 1), random allocation (item 3), blinded assessment (item 9), and losses and exclusions (item 11), the first two were commonly satisfied (in 15 and 17 papers, respectively), whereas using unbiased/blind/naïve study subjects and accounting for losses and exclusions were ignored in many studies (present in 7 and 5, respectively). Five of the reviewed articles ([18, 23, 36, 43, 44]) should be considered with care since their assessments of outcomes were not blinded.

Seven articles did not mention if the allocation of study subjects to each group was randomized. As it was not our intention to penalize unfairly articles that used a randomized allocation without mentioning it, we identified the numbers of individuals in each group in these reports (see Table 3). Articles [19, 32, 33] did not provide the number of subjects in each experimental group. The others (except [30]) showed a more even distribution than one could expect with a proper randomization procedure. We therefore assumed that the fact that authors did not discuss the randomization procedure, in most cases, reflected the fact that it had not occurred.

In our review, we did not set a threshold for acceptable/unacceptable quality but displayed the articles in descending order from the highest to the lowest score. Nevertheless, in Table 2a, we compared the spread of articles when using the total score (18 point scale, see Table 2) and the scale based on four high-priority items (8 point scale, see Table 2b). With levels of “acceptance” provisionally set at 12/18 and 4/8, respectively, all articles, with one exception, obtained the same classification of acceptable or not, regardless of the system used. We interpreted this concordance as a confirmation of the robustness of the quality and risk of bias assessment.

In addition to the quality issues identified in this review, the authors themselves have in some instances discussed the limitations of their own studies (see Table 4).

Table 2 a.   A comparison between total scores
obtained from two different scales
(8 point scale and 18 point scale)

Table 2 b.   Four main quality criteria of articles
from literature review on effect of SMT on pain
– maximum score 8 points

A meta-analysis was not attempted because the heterogeneity of studies on pain provoking methods, units of measurements, areas of the body where experiments were performed, and local, regional of systemic assessments of the effects of SMT.

      Data synthesis: Answers to research questions

Herein, each research question is dealt with one at a time, and for each, we refer to a table summarizing the results in order of quality of the study. If one study contains several experiments, they have all been reported individually in the appropriate section of the review. Table 5 presents studies grouped by their results, showing in the upper panels those that presented a hypoalgesic action and, in the lower panels, those that did not. Papers are also separated by the research question, i.e. local, regional or systemic effect. Table 6 summarizes for each article the effect of SMT on pain, the site where SMT was performed and the location of the pain. This table also shows values reported, whether the effect was local, regional or systemic, whether the effects were ipsilateral or contralateral, whether an effect occurred above or below the site of manipulation, and the type of pain induced.

In the majority of experiments (28/43), SMT reduced pain. Outcomes were not dependent on whether quality scores were high or low. These studies demonstrate a clear hypoalgesic effect of SMT (see Table 5a). For specific results, see below.

Table 3.   Distribution of subjects in study groups
in articles where allocation procedure was not specified

Table 4.   Limitations to own studies given by authors
of articles reviewed for the effect of SMT on pain

Table 5.   Effect of SMT on experimentally induced
pain by localization of pain reduction

Table 5a.   Effect of SMT on experimentally induced
pain by localization of pain reduction in relation to
the quality of studies

For specific results, see below.

  1. Does SMT reduce experimentally induced pain provoked by pressure? There were 27 experiments performed on pressure pain. Nineteen of these showed manipulation to increase pressure pain thresholds (PPT). There was no obvious link between the reported polarity of the effect and the quality of the studies. Changes produced by SMT were in general statistically significant. Differences in PPT values (before/after SMT), when reported as percentages ranged between 4.8% (in [28]) and 44.2% (in [19]). Other units of measurement were also used making it difficult to summarize with a single parameter the size of the effect. For detailed information, see Tables 6 and Additional file 3.

    Table 6.   Detailed findings from literature review on effect of SMT on pain

  2. Does SMT reduce experimentally induced pain provoked by temperature? There were 9 experiments on pain induced by temperature, only 3 of which showed a hypoalgesic action for SMT: studies [18, 23, 36] found that SMT relieves pain provoked by temperature and therefore transmitted by C-fibers. The other experiments (n?=?6) did not show significant differences in relation to SMT. Three of these tested first pain (A fiber system) and the other three tested TSS (C-fiber system). There was no obvious link with the quality of studies. For values and detailed information, see Table 6 and Additional file 4.

  3. Does SMT reduce experimentally induced pain provoked by methods other than pressure and temperature? Only 7 tests were performed using methods other than pressure and temperature to induce pain. Six of these revealed a statistically significant hypoalgesic effect induced by SMT. There did not seem to be a link with the quality of studies. For values and detailed information, see Tables 6 and Additional file 5.

  4. Does SMT reduce experimentally induced pain in the spinal segment where it is performed? Twenty experiments investigated whether SMT reduces experimentally induced pain in the spinal segment where it is performed. Twelve of them showed a hypoalgesic effect. The other eight presented no significant effects. Of these, study [28] tested the hypoalgesic action of an anterior-posterior mobilization and not a lateral maneuver, as is usually the case in SMT. In study [20], there was no effect immediately after SMT, but an effect was demonstrable five and ten minutes later. In studies [19, 31] differences between sides were studied, but no difference was found. Outcomes did not relate to the quality score. For detailed information, see Tables 5 and 6.

  5. Does SMT reduce experimentally induced pain in the same region where it is performed? Nine experiments reported a regional effect of SMT on pain. Only one study failed to obtain a hypoalgesic effect. In this, thoracic manipulation was used as a sham treatment versus cervical manipulation to evaluate the action on elbow PPT. There did not seem to be a link between treatment effects reported and the quality of studies. For detailed information, see Table 5 and 6.

  6. Does SMT have a systemic effect on experimentally induced pain? Nine experiments evaluated this hypothesis. None of them had blinded assessors, which makes the results uncertain. Five of them demonstrated a systemic action of SMT on pain, but four of them did not show significant differences between treatment groups. Three of these four (studies [18, 23, 36]) evaluated first pain transmitted by A fibers. There did not seem to be a link between treatment effects reported and the quality of studies. For detailed information, see Tables 5 and 6.

      Additional observations

  1. No article presented any data on the duration of the pain reduction. Studies [43, 44] concluded that the hypoalgesic effect was unrelated to the amplitude of the manual procedure, whereas [30] concluded that mobilization had a stronger effect than manipulation.

  2. Four studies ([29, 35, 36, 40]) applied the SMT to a point on the spine thought to be in need of treatment, i.e. at what was considered to be a dysfunctional spinal segment (manipulable lesion/fixation/subluxation), whereas in all other studies the exact location of SMT was predetermined without reference to local signs or symptoms. However, hypoalgesic results were observed regardless of whether the treatment was provided in a “clinical” fashion, i.e. where the patient would have what the clinician considered a dysfunctional segment, or if the manipulation was given in a predetermined area.

  3. In two studies ([20, 29]) the investigators searched for so-called trigger points.

  4. Five articles ([19, 31, 37, 39, 42]) assessed whether the effect of SMT was only on the ipsilateral side of the impulse or if it was also noted on the contra-lateral side. None of the trials found a side-specific effect.

  5. Several reports (N?=?9) made reference to the “crack” ([18-20, 23, 29, 37-39, 42]), which is thought to occur when a joint is cavitated [14]. If no cavitation was obtained, a second manipulation would be given. There was no obvious difference in results between studies that concentrated on the crack and those that did not. However, none studied that issue specifically, making it impossible to know whether all study subjects were “cracked” or not.

  6. Regarding whether the effect could be noticed above or below the segment where the SMT was performed, results lacked consistency. In two of the studies ([23, 39]), an effect was noted below the area of treatment. Studies [39, 44] showed an effect above the area of treatment, whereas two of the studies ([36, 37]) did not detect an effect above or below the relevant segment.

  7. Outcomes were not affected by whether the control group received a sham treatment or no treatment at all.

  8. In 10 of the experiments (5 articles), patients with current pain were included ([18, 19, 21, 32, 33]). Three of these experiments ([19, 21, 32]) found increased PPT values after SMT. Article [33] showed relief of pain on stretching painful muscles after SMT but not relief of spontaneous pain, whereas article [21] reported that spontaneous pain was diminished by SMT. Articles [18, 19] evaluated temperature-induced pain with similar results, that is to say there was a hypoalgesic action on second pain [18], no action on first pain [18] and no action in 2 trials on TSS.


      Summary of results

At the time of conducting this review, only one earlier systematic review was available on this subject. It examined 11 articles but was unable to draw conclusions regarding treatment effects [45]. For the present review, however, it was possible to identify 22 relevant articles, which made it possible to draw several conclusions in relation to the possible pain-reducing effect of SMT. Thus our results indicate clearly that such an effect is achievable.

However, differences in effect exist according to whether the outcome is tested locally, regionally or systemically. An effect was clearly shown locally and regionally, whereas an effect is less clear in more distant parts of the body. Also the outcome differs according to the method of pain induction; pain induced by pressure, electricity, stretching of painful tissue, dermal irritation, and spontaneous pain all respond to SMT, whereas temperature-induced pain does not always respond.

      Methodological considerations of our own review

One limitation of this review is that there is no generally accepted and validated quality check list for the type of experimental studies which we examined and so we had to select our own quality criteria, based on some basic methodological concepts important to our research questions. Such a check list can be modified, which could affect the overall quality assessment. According to our quality scoring system, studies scored between 8 and 16 points out of a possible 18. However, in order to discriminate better between studies, a more detailed scale could have resulted in different scores, which could perhaps have separated studies into more obviously “good” and “bad”. However, the relevance, if any, of these quality items in relation to outcome, is not known and, in fact, the quality scores were not clearly associated with outcomes.

The strengths of this review are that the search for relevant articles was free of language bias, that the constituent articles were reviewed independently by two reviewers, that the results can be considered in relation to the quality of studies, that there was no arbitrary threshold for acceptable quality [46], that the check list tables are sufficiently detailed to allow readers to perform their own analysis of the information provided, and that there was a relatively large number of studies, making it possible to examine several research questions.

      Methodological considerations for the studies under review

This review identified some common methodological problems, such as lack of blinded assessment that could weaken the evidence in these experimental studies. We considered one of the most important points to be that the assessor was blinded, to avoid expectation bias. Some studies ignored this issue. This aspect affected our interpretation of the systemic effect of SMT, as all five articles (10 trials) dealing with the systemic effects of SMT lacked a blinded assessor.

It is also important that the results be truthfully presented, and not exaggerated in some way, meaning that any subjects or data excluded from analysis should be accounted for. This was often ignored.

Another challenge was that of the control group. Ideally, the SMT should be matched against a suitable sham treatment and control procedure, and this was done in 12 studies. This is difficult with physical procedures, but several of the other 10 studies tried to make the best of the situation by selecting naive study subjects, and in some cases different types of treatment were compared (such as treatment in two areas of the spine), in which case no sham treatment would be necessary. However, whether a proper sham treatment was used or not, there were no obvious differences in the results, possibly indicating that the effect is very obvious and not affected to a large extent by expectations.

Potential confounders of these effects would be anxiety in general and fear of pain, known moderators of treatment outcomes in clinical practice. However, according to the three articles in which anxiety and fear of pain were studied, there were no statistically significant correlations between pain-related cognition, pain thresholds and the hypoalgesic response to SMT, indicating that the psychological aspect, considered to be so important in the perception of pain, perhaps does not come into play during experimental studies of this type.

      Comparison with another systematic review

Some of our results were corroborated by a newly published high quality review on this very topic [47]. This other review included 15 of our articles but, as they did not put a restriction on duration of symptoms in symptomatic people, they also incorporated 5 articles not included in our review. They defined effects as occurring “locally” or in “remote” areas, and pooled the results in all studies for the effects of PPT. They found the effects on PPT to be small in both areas but nevertheless statistically significant. When the data were separated for local and remote effect significance was only noted for the remote effect. They did not study any other pain–inducing method nor did they take into consideration the quality of studies, although they did perform a quality assessment.

      Discussion of findings

Pain provoked by pressure, electricity, stretching of painful tissue, dermal irritation, or spontaneous pain all respond to SMT.

Experimental pain provoked by pressure was the most common method used to assess the effect of manipulation (27 out of 43 experiments). Unfortunately, the reports did not use the same units for reporting increased tolerance to pressure, making it difficult to compare outcomes, but SMT was shown to increase PPT values between 4.8% and 44.6%. That the algometer, used for this purpose, is a highly reliable method to measure pain [48] suggests that these results are robust. However, they cannot necessary be compared between studies because of different experimental situations.

Nevertheless, it is not clear whether these changes are clinically significant. One author [44] referred to Moss [49], who stated that a 15% improvement in pressure pain tolerance is clinically important, but this statement rests on previous studies which dealt with osteoarthritic pain [50] and pain in an emergency service [51], not experimental pain. Therefore, the cut-off point at which the reduction of artificially induced pain has reached a clinically significant level is not known.

In addition, it cannot be concluded that the effect of SMT, as seen in these studies, would be as large in "naturally" painful areas. Therefore, it is not certain that the degree of pain reduction detected in experimental studies can be extrapolated to other situations.

Apart from pressure and temperature, other sources of pain were: capsaicin to irritate the skin followed by skin stroking to evoke allodynia, hyperalgesia induced by mechanical means, and spontaneous pain. In these instances also, SMT was generally able to reduce pain.

Pain provoked by temperature does not always respond to SMT.

Nine trials from five papers examined the effects of SMT on temperature induced pain. In 3 of the 6 tests examining second pain, an effect was found but none of the 3 trials evaluating first pain could demonstrate such an effect. This indicates that SMT may have an effect on C-fiber mediated “second pain” but not on the more acute “first pain” mediated by A-delta fibers. This finding may help to resolve the mechanisms by which SMT reduces pain.

How broadly does the effect extend?

Almost all authors discussed the possibility of a systemic effect of SMT but this was tested in only 5 studies (9 experiments). The results were clearly positive, although two of the authors concluded that local effects were stronger than more distant ones. However, none of these studies used a blinded assessor, and so the reported results must be treated with caution.

Nine experiments out of the ten that studied the regional effect were positive (see Table ?Table5).5). However, in the reviewed articles, it was difficult to differentiate between strictly local vs. more regional effects. The definition of regional is uncertain if following the dermatomes, as they are known to differ from the text-book mappings [52] and the origins and distributions of cutaneous nerves differ from person to person. Thus, a negative outcome in one person may simply be due to that person having an unusual pattern of nerve distribution. Despite this, most studies showed a positive effect with relatively few study subjects. This could indicate either that negative results were removed from the analyses or that the effect is consistent.

Imprecise dermatomal mapping makes it difficult not only to separate clearly local from regional effects but also regional from systemic effects. Interestingly, the application of spinal manipulation at the atlanto-occipital region was found to have an effect on the masseter muscle, despite that muscle being supplied by the trigeminal nerve, which does not exit in the upper cervical spine. We therefore described this experiment under “regional” rather than “local” but it could perhaps equally well have been described it as “systemic”.

Concerning the action of SMT on pain produced above or below the manipulated segment, only a few studies dealt with this and no consistent findings emerged. It would be necessary to test this issue specifically, in order to work out whether manipulation-induced impulses travel up or down the spinal cord or whether both occur.

Five studies investigated whether the hypoalgesic effect was mainly on the side of the manipulation or if it also appeared on the opposite side. These studies consistently demonstrated a bilateral effect.

Some clinical concepts

Although this review was based on experimentally induced pain, four findings emerged that could have a bearing on clinical practice, or at least on the concepts on which clinical practice is based. Manipulation of a “restricted motion segment”, sometimes referred to as a “manipulable lesion”, was not required for the “treatment” to have an effect. Additionally, the side of the manipulation was irrelevant. However, the results from this review can only be considered from a clinical point of view, if SMT has a similar effect on pain in a clinical context, which was not investigated in this study.

Finally, although a hypoalgesic effect was shown, it is not known how long this effect lasts; long enough for a person with pain to be able to regain a normal movement pattern or only long enough to give an impression of improvement? Other effects of the SMT were not studied in this review; effects such as improved biomechanics or reduced inflammation of disturbed tissues [53].

      Implications in relation to research

There is a need to establish a coordinated global research strategy on the subject of SMT and pain relief. It seems unnecessary to conduct further research on simply whether SMT has an effect on pain or not. Rather, future work should focus on more precise questions such as why SMT does not affect first pain, and which mechanisms are involved in relieving pain locally, regionally and systemically. The magnitude and duration of effects also need to be defined. Consideration needs to be given to the most appropriate research designs for addressing different questions.

There could also be more consistency in outcome measures, and closer attention should be paid to important design elements such as blinded assessment, random allocation, appropriate control groups and, if possible, the recruitment of naïve subjects.

      Implications in relation to education and clinical practice

Some of the findings in this review do not support the imperative of specificity, i.e. precise identification of a manipulable lesion and the exact level and side of the manipulation.


This systematic critical review of the literature confirms an effect of spinal manipulative therapy (SMT) on experimentally induced pain in human beings. This hypoalgesic effect seems to be local/regional and more consistent for pain provoked by pressure than by temperature. Further and better research is needed to determine if there is a systemic effect, to determine the exact mechanisms by which SMT relieves pain, the clinical importance and duration of the hypoalgesic effect.

Competing interests

The authors declare that they have no competing interests.

Author’s contributions

All authors instigated this review. MM and CLY designed the check-lists, reviewed the literature and wrote the first draft. BB and MAA provided expertise on the topic, assisted with the literature review and provided critical comments to the first draft. All authors reviewed the final manuscript and approved the final version.

Supplementary Material

Additional file 1:   Inclusion and exclusion criteria in a survey of the effect of spinal manipulative therapy on experimentally induced pain [2, 46, 54-60]

Additional file 2:   The items selected for the quality checklist and their rationale.

Additional file 3:   Effects of SMT on pressure pain thresholds (PPT).

Additional file 4:   Effects of SMT on pain produced by temperature.

Additional file 5:   Effects of SMT on pain produced by methods other than pressure or temperature.


Søren O´Neill, DC for helping at the stage of conception and generously sharing his insights into technical matters.


  1. International Association for the Study of Pain Taxonomy.…isplay.cfm&ContentID=1728#Pain

  2. Kandel ESJ, Jessell T.
    Principles of neural science, vol. International.
    McGraw-Hill, Fourth edn; 2000

  3. Tandon OP, Malhotra S, Tandon S, D'Silva I.
    Neurophysiology of pain: insight to orofacial pain.
    Indian J Physiol Pharmacol. 2003;47(3):247–269

  4. Wall M. In: 5th edn. Mahon SM, editor. Elservier; 2006.
    Wall and Melzack's textbook of pain; pp. 3–201

  5. Ji R, Kohno T, Moore KA, Woolf CJ.
    Central sensitization and LTP: do pain and memory share similar mechanisms?
    Trends Neurosci. 2003;26(12):696–705

  6. Mense S, Gerwin R.
    Muscle Pain: Understanding the Mechanisms.
    Springer; 2010. Central nervous mechanisms of muscle pain: ascending pathways, central sensitization, and pain-modulating systems; pp. 126–144

  7. Rhudy JL, Williams AE, McCabe KM, Nguyen M, Rambo P.
    Affective modulation of nociception at spinal and supraspinal levels.
    Psychophysiology. 2005;42(5):579–587

  8. Villemure C, Bushnell MC.
    Cognitive modulation of pain: how do attention and emotion influence pain processing?
    Pain. 2002;95(3):195–199

  9. Walter H.
    The biomechanics of spinal manipulation.
    J Bodyw Mov Ther. 2010;14(3):280–286

  10. Maigne J-Y, Vautravers P.
    Mechanism of action of spinal manipulative therapy.
    Joint Bone Spine. 2003;70(5):336–341

  11. Vernon H, Mrozek J.
    A Revised Definition of Manipulation
    J Manipulative Physiol Ther 2005 (Jan); 28 (1): 68–72

  12. Flynn T, Fritz J, Wainner R, Whitman J.
    The audible pop is not necessary for successful spinal high-velocity thrust manipulation in individuals with low back pain.
    Arch Phys Med Rehabil. 2003;84:1057–1060

  13. Flynn T, Childs J, Fritz J.
    The audible pop from high-velocity thrust manipulation and outcome in individuals with low back pain.
    J Manipulative Physiol Ther. 2006;29:40–45

  14. Cramer G, Ross K, Pocius J, Cantu J, Laptook E, Fergus M, Gregerson D, Selby S, Raju P.
    Evaluating the Relationship Among Cavitation, Zygapophyseal Joint Gapping,
    and Spinal Manipulation: An Exploratory Case Series

    J Manipulative Physiol Ther. 2011 (Jan);   34 (1):   2–14 ~ FULL TEXT

  15. Cleland J, Flynn T, Childs J, Eberhart S.
    The audible pop from thoracic spine thrust manipulation and its relation to short-term outcomes in patients with neck pain.
    J Man Manipulative Ther. 2007;15(3):143–154

  16. Bialosky JE, Bishop MD, Robinson ME, George SZ.
    The relationship of the audible pop to hypoalgesia associated with high-velocity, low-amplitude thrust manipulation: a secondary analysis of an experimental study in pain-free participants.
    J Manipulative Physiol Ther. 2010;33(2):117–124

  17. Clark B, Goss D, Walkowski S, Hoffman R, Ross A, Thomas J.
    Neurophysiologic Effects of Spinal Manipulation in Patients With Chronic Low Back Pain
    BMC Musculoskelet Disord. 2011 (Jul 22); 12: 170

  18. Bialosky JE, Bishop MD, Robinson ME, Zeppieri G, Jr, George SZ.
    Spinal Manipulative Therapy Has an Immediate Effect on Thermal Pain Sensitivity
    in People With Low Back Pain: A Randomized Controlled Trial

    Phys Ther. 2009 (Dec); 89 (12): 1292–1303

  19. Fernández-Carnero J, Fernández-de-las-Peñas C, Cleland J.
    Immediate hypoalgesic and motor effects after a single cervical spine manipulation in subjects with lateral epicondylalgia.
    J Manipulative Physiol Ther. 2008;31(9):675–681

  20. Ruiz-Saez M, Fernandez-de-las-Penas C, Blanco CR,
    Martinez-Segura R, Garcia-Leon R.
    Changes in Pressure Pain Sensitivity in Latent Myofascial Trigger Points in the
    Upper Trapezius Muscle After a Cervical Spine Manipulation in Pain-Free Subjects

    J Manipulative Physiol Ther. 2007 (Oct); 30 (8): 578–583

  21. Sterling M, Jull G, Wright A.
    Cervical mobilisation: concurrent effects on pain, sympathetic nervous system activity and motor activity.
    Man Ther. 2001;6(2):72–81

  22. Joel GP.
    Neurophysiological Effects of Spinal Manipulation
    Spine J (North American Spine Society) 2002 (Sep); 2 (5): 357–671

  23. Bishop MD, Beneciuk JM, George SZ.
    Immediate reduction in temporal sensory summation after thoracic spinal manipulation.
    Spine J. 2011;11(5):440–446

  24. Bialosky JE, Bishop MD, Price DD, Robinson ME, George SZ.
    The Mechanisms of Manual Therapy in the Treatment of
    Musculoskeletal Pain: A Comprehensive Model

    Man Ther. 2009 (Oct);   14 (5):   531–538

  25. Liberati A, Altman D, Tetzlaff J, Mulrow C, Gøtzsche P, Ioannidis J, Clarke M, Devereaux P, Kleijnen J, Moher D.
    The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration.
    J Clin Epidemiol. 2009;62(10):e1–e34

  26. Moher D, Hopewell S, Schulz K, Montori V, Gøtzsche P, Devereaux P, Elbourne D, Egger M, Altman D.
    CONSORT 2010 explanation and elaboration: updated guidelines for reporting parallel group randomised trials.
    Int J Surg. 2011;1:27–54

  27. Furlan A, Pennick V, Bombardier C, Van Tulder M.
    2009 updated method guidelines for systematic reviews in the Cochrane back review group.
    Spine. 2009;34(18):1929–1941

  28. Soon B, Schmid A, Fridriksson E, Gresslos E, Cheong P, Wright A.
    A crossover study on the effect of cervical mobilization on motor function and pressure pain threshold in pain-free individuals.
    J Manipulative Physiol Ther. 2010;33(9):652–658

  29. Oliveira-Campelo N, Rubens-Rebelatto J, Marti N, Vallejo F, Alburquerque-Sendi N, Fernandez-de-Las-Penas C.
    The immediate effects of atlanto-occipital joint manipulation and suboccipital muscle inhibition technique on active mouth opening and pressure pain sensitivity over latent myofascial trigger points in the masticatory muscles.
    J Orthop Sports Phys Ther. 2010;40(5):310–317

  30. Thomson O, Haig L, Mansfield H.
    The effects of high-velocity low-amplitude thrust manipulation and mobilisation techniques on pressure pain threshold in the lumbar spine.
    Int J Osteopathic Med. 2009;12:56–62

  31. Fernandez-de-las-Penas C, Perez-de-Heredia M, Brea-Rivero M, Miangolarra-Page JC.
    Immediate Effects on Pressure Pain Threshold Following a Single Cervical
    Spine Manipulation in Healthy Subjects

    J Orthop Sports Phys Ther. 2007 (Jun);   37 (6):   325–329

  32. Vicenzino B, Collins D, Benson H, Wright A.
    An investigation of the interrelationship between manipulative therapy-induced hypoalgesia and sympathoexcitation.
    J Manipulative Physiol Ther. 1998;21(7):448–453

  33. Vicenzino B, Collins D, Wright A.
    The initial effects of a cervical spine manipulative physiotherapy treatment on the pain and dysfunction of lateral epicondylalgia.
    Pain. 1996;68(1):69–74

  34. Hamilton L, Boswell C, Fryer G.
    The effects of high-velocity, low-amplitude manipulation and muscle energy technique on suboccipital tenderness.
    Int J Osteopathic Med. 2007;10:42–49

  35. G, Carub J, McIver S.
    The effect of manipulation and mobilisation on pressure pain thresholds in the thoracic spine.
    J Osteopathic Med. 2004;7(1):8–14

  36. George SZ, Bishop MD, Bialosky JE, Zeppieri G, Robinson ME.
    Immediate effects of spinal manipulation on thermal pain sensitivity: an experimental study.
    BMC Musculoskelet Disord. 2006;7(1):68

  37. Fernández-Carnero J, Cleland JA, Arbizu RLT.
    Examination of motor and hypoalgesic effects of cervical vs thoracic spine manipulation in patients with lateral epicondylalgia: a clinical trial.
    J Manipulative Physiol Ther. 2011;34(7):432–440

  38. Mansilla-Ferragut P, Fernández-de-las Peñas C, Alburquerque-Sendín F, Cleland JA, Boscá-Gandía JJ.
    Immediate effects of atlanto-occipital joint manipulation on active mouth opening and pressure pain sensitivity in women with mechanical neck pain.
    J Manipulative Physiol Ther. 2009;32(2):101–106

  39. Fernández-de-las-Peñas C, Alonso-Blanco C, Cleland J, Rodríguez-Blanco C, Alburquerque-Sendín F.
    Changes in pressure pain thresholds over C5-C6 zygapophyseal joint after a cervicothoracic junction manipulation in healthy subjects.
    J Manipulative Physiol Ther. 2008;31(5):332–337

  40. Mohammadian P, Gonsalves A, Tsai C, Hummel T, Carpenter T.
    Areas of Capsaicin-Induced Secondary Hyperalgesia and Allodynia
    Are Reduced by a Single Chiropractic Adjustment: A Preliminary Study

    J Manipulative Physiol Ther. 2004 (Jul);   27 (6): 381–387

  41. Terrett A, Vernon H. Manipulation and pain tolerance.
    A controlled study of the effect of spinal manipulation on paraspinal cutaneous pain tolerance levels.
    Am J Phys Med. 1984;63(5):217–225

  42. Maduro de Camargo V, Alburquerque-Sendín F, Bérzin F, Cobos Stefanelli V, Rodrigues de Souza D, Fernández-de-las-Peñas C.
    Immediate effects on electromyographic activity and pressure pain thresholds after a cervical manipulation in mechanical neck pain: a randomized controlled trial.
    J Manipulative Physiol Ther. 2011;34(4):211–220

  43. Krouwel O, Hebron C, Willett E.
    An investigation into the potential hypoalgesic effects of different amplitudes of PA mobilisations on the lumbar spine as measured by pressure pain thresholds (PPT)
    Man Ther. 2010;15(1):7–12

  44. Willett E, Hebron C, Krouwel O.
    The initial effects of different rates of lumbar mobilisations on pressure pain thresholds in asymptomatic subjects.
    Man Ther. 2010;15(2):173–178

  45. Vernon H.
    Qualitative Review of Studies of Manipulation-induced Hypoalgesia
    J Manipulative Physiol Ther 2000 (Feb); 23 (2): 134–138

  46. Chesterton L, Sim J, Wright C, Foster N.
    Interrater reliability of algometry in measuring pressure pain thresholds in healthy humans, using multiple raters.
    Clin J Pain. 2007;23(9):760–766

  47. Coronado RA, Gay CW, Bialosky JE, Carnaby GD, Bishop MD, George SZ.
    Changes in pain sensitivity following spinal manipulation: a systematic review and meta-analysis.
    J Electromyogr Kinesiol. 2012

  48. Chesterton LSS, Wright J, Christine C, Foster NE.
    Interrater Reliability of Algometry in Measuring Pressure Pain Thresholds in Healthy Humans, Using Multiple Raters.
    Clin J Pain. 2007;23(9):760–766

  49. Moss P, Sluka K, Wright A.
    The initial effects of knee joint mobilization on osteoarthritic hyperalgesia.
    Man Ther. 2007;12(2):109–118

  50. Bird SB, Dickson EW.
    Clinically significant changes in pain along the visual analog scale.
    Ann Emerg Med. 2001;38(6):639–643

  51. Bellamy N, Bell MJ, Goldsmith CH, Pericak D, Walker V, Raynauld JP, Torrance GW, Tugwell P, Polisson R.
    Evaluation of WOMAC 20, 50, 70 response criteria in patients treated with hylan G-F 20 for knee osteoarthritis.
    Ann Rheum Dis. 2005;64(6):881–885

  52. Downs MB, LaPorte C.
    Conflicting dermatome maps: educational and clinical implications.
    J Orthop Sports Phys Ther. 2011;41(6):427–434

  53. Leach RA.
    In: Fourth edn. Darcy P, editor. Lippincott Williams & Wilkins; 2004.
    The chiropractic theories; pp. 131–197–235–246

  54. Schmelz M.
    Translating nociceptive processing into human pain models.
    Exp Brain Res. 2009;196(1):173–178

  55. Modir J, Wallace M.
    Human experimental pain models 2: the cold pressor model.
    Analgesia - Meth Mol Biol. 2010;617:165–168

  56. Graven-Nielsen T, McArdle A, Phoenix J, Arendt-Nielsen L, Staehelin Jensen T, Jackson MJ, Edwards RHT.
    In vivo model of muscle pain: Quantification of intramuscular chemical, electrical, and pressure changes associated with saline-induced muscle pain in humans.
    Pain. 1997;69(1–2):137–143

  57. Graven-Nielsen T, Fenger-Gron LS, Svensson P, Steengaard-Pedersen K, Arendt-Nielsen L, Jensen TS.
    Quantification of deep and superficial sensibility in saline-induced muscle pain-a psychophysical study.
    Somatosens Mot Res. 1998;15(1):46–53

  58. Modir J, Wallace M.
    Human experimental pain models 3: heat/capsaicin sensitization and intradermal capsaicin models.
    Analgesia - Meth Mol Biol. 2010;617:169–174

  59. Müller H.
    Neuroplasticity and chronification of pain.
    Anästhesiology Intensivmedizin Notfallmed Schmerztherapie. 2000;35(05):274–284

  60. Zhuo M.
    Central plasticity in pathological pain.
    Novartis Found Symp. 2004;261:132–145. discussion 145–5



Since 9-29-2015

         © 1995–2018 ~ The Chiropractic Resource Organization ~ All Rights Reserved