Zhongguo Yao Li Xue Bao 1997 (Jul); 18 (4): 344–347
Zhu L, Wu J, Liao H, Gao J, Zhao XN, Zhang ZX
School of Medicine,
Nanjing University, China
To determine whether the extract of leaves
of Ginkgo biloba L (EGb) and several active constituents of
EGb have protective effects against glutamate (Glu)–induced
METHODS: Microscopy and image analysis of
nucleus areas in the arcuate nuclei (AN) of mice were made.
The neuronal viability in primary cultures from mouse
cerebral cortex was assessed using MTT [3-(4,
5-dimethylthiazol-2-yl)– 2, 5-diphenyl tetrazolium bromide]
staining and the intracellular free calcium concentration
([Ca2+]i) of single neuron was measured using Fura–2.
RESULTS: EGb (2.5 mg.L–1) and its constituent ginkgolide B
(Gin B, 2 mg.L-1) protected the neuronal viability against
Glu-induced injury, and prevented the Glu–induced elevation
in [Ca2+]i. EGb (3-10 mg.kg–1) attenuated the decrease of
nucleus areas in arcuate nuclei induced by Glu (1 g.kg–1,
CONCLUSION: EGb and Gin B prevent neurons from Glu
neurotoxicity through reduction of the rise in [Ca2+]i.